Effect of Gemzar drug and plant extract of Crocus sativus L on some antioxidants in male rats

Authors

  • Safa Masser kmosh Community health technologies department, Technical Institute of Al-Diwaniyah, AL-Furat AL Awsat Technical University, Al-Diwaniya, Iraq*
  • Masar J. AL-Kurdy Community health technologies department, Technical Institute of Al-Diwaniyah, AL-Furat AL Awsat Technical University, Al-Diwaniya, Iraq*
  • Malath I. Yousif Community health technologies department, Technical Institute of Al-Diwaniyah, AL-Furat AL Awsat Technical University, Al-Diwaniya, Iraq

Keywords:

Extract Of Saffron, Gemzar, Oxidative Stress, Antioxidant

Abstract

The current study was designed to evaluate the antioxidant effects of the chemical drug "Gemzar". In addition to study the role of the aqueous extract of the stigma of plant saffron to decrease the toxicity of Gemzar. In this study, 30 white adult rats aged 9-12 weeks were grouped into five groups (6 rats in each group ). The first group was negative control which received normal saline for four weeks. The second group was positive control received Gemzar in a dosage 15 mg/kg of body weight for four weeks. The third group was orally received a plant extract at a dosage of (100) mg/kg of body weight before medication for two weeks, then received drug for two weeks. The fourth group was received both plant extract (100) mg/kg and the drug for four weeks. The fifth group was received plant extract two weeks after medication. The experiment was maintained for four weeks, then the rats were killed, and the blood was drawn to study the pathological effects in the examined parameters. The statistical analysis showed a considerable decrease in catalase, albumin, with increasing of lipid peroxide levels in the group treated with drug in comparison with control group. However, the treatment with both plant extract 100 mg/kg and the drug was administered in three treatments with a plant extract (before, during, and after), the drug receiving. The current study showed a significant improvement in some cases of negative control. It may revealed that the exposure to Gemzar caused alterations in biochemical indicators of rats blood. The current study improved that that the uptake of the plant extract simultaneously with the drug or (before and after) the drug has a significant role to increasing the harm consequences caused by the drug.

References

Ahn, H., Hwang, K., Hong, S., Yang, D., Lee, B., Todd, A.(2000). Chronic lead nephropathy with excessive body lead burden. J Occup Health. 2:260–262.

Al-Mansour, Nasser Abd Ali. (1999). Evaluation of the efficiency of some plant extracts in affecting the hatching and mortality of mosquitoes Culex quinquefasciatns Say (Diptera: culicidae). Basra Journal of Agricultural Sciences 12(2).183-193.

Aebi, H. (1974) " Methods in Enzymatic Analysis Bergmeyer ".(ed). Verlay chem. Wrinheim ; 673.

Agramonte, M. D. A. R.; Gonçalves, C. A. G. and. Siniscalco, D.(2019). Selected Papers from CUBANNI-2017“The Fourth International Workshop of Neuroimmunologi" published in behavioral Science. Pp:53-54. https://doi.org/10.3390/books978-3-03897-488-8

Basha, B. and Sovers, I. (1996). Atherosclerosis: an update. Am. Heart. J., 131, 1192- 1202. DOI: 10.1016/s0002-8703(96)90096-4

Bendich, A. (1989). Carotenoids and the immune response. J. Nutr. 119: 112-115. DOI: 10.1093/jn/119.1.112

Cusano E, Consonni R, Petrakis EA, Astraka K, Cagliani LR, Polissiou MG.Integrated analytical methodology to investigate bioactive compounds inCrocus sativus L. flowers. Phytochem Anal. 2018;29(5):476-86

(PDF) Effect of Afghan Saffron (Crocus sativus L.) Aqueous Extract on Withdrawal Signs in Morphine-Dependent Rats. Available from: https://www.researchgate.net/publication/345251562_Effect_of_Afghan_Saffron_Crocus_sativus_L_Aqueous_Extract_on_Withdrawal_Signs_in_Morphine-Dependent_Rats [accessed Feb 19 2023].

Chitkara, D. , Anupama, M. ,Stephan, W., Behrman ,N. K., and RamI,M.(2013). Self-Assembling,Amphiphilic Polymer- Gemcitabine Conjugate Shows Enhanced Antitumor Efficacy against Human Pancreatic Adenocarcinoma. Bioconjugate chemistry24(7):1161-73. DOI: 10.1021/bc400032x

Daniel, W. W. and Cross, C. L. (2018). Biostatistics: a foundation for analysis in the health sciences.Wiley.19-750.

Dog, L.W.(2006). Areason to season: the therapeutic benefits of spices and culinary herbs . Diet and Nutrition 2(5),446-449. DOI: 10.1016/j.explore.2006.06.010

EL-Maragy, S. A, Rizk, S.A. and EL-Sawalhi, M.M.(2009). Hepatoprotective potential of crocin and curcumine against iron overload- iduced biochemical alterations in rat. Arf. J. Biochem. Res.3(5): 215-221.

Goli, S. A. Mokhtari, F. and Rahimmalek, M.(2012). Phenolic Compounds and Antioxidant Activity from Saffron (Crocus sativus L) Petal. J Agr Sci 4:175-181.

Halliwell, B.; Gutteridge, J.M.C. (1999). "Free Radical in Biology and Medicine".3rd edn.Oxford, Oxford University Press, pp.146-163, 399-430.

Hassan, S. M.; Al-Kennany, E. R.; Al-Hafez, H. A. (2000). Hydrogen peroxide- induced atherosclerosis in chicken effect of vitamin C. Iraqi J. Vet., 13, 249-270.

Hosseinzadeh, H. and Nassiri, A. M.(2013). Avicenna’s (Ibn Sina) the Canon of Medicine and Saffron (Crocus sativus): A Review. Phytother Res 27:475-483. DOI: 10.1002/ptr.4784

Ji, J., Liu, J., Liu, H., Wang, Y. (2014). Effects of fermented mushroom of cordyceps sinensis, rich in selenium, on uterine cervix cancer. Evid Based Complement Alternat Med.1(2):1-12. DOI: 10.1155/2014/173180

Karimi, E., Oskoueian, E., Hendra, R. and Jaafar, H.Z.(2010). Evaluation of Crocus Sativus L. Stigma phenolic and flavonoid compounds and its antioxidant activity .Molecules 15:6244- 6256. DOI: 10.3390/molecules15096244

Kharb, M.,Jat, R.K. ,Gupta, A (2012). A review on medical plants used As a source of anticancer agents. Int J Drug Res Technol 2:177-183.

Liu, F.S.(2009). Mechanisms of chemotherapeutic drug resistance in Cancer therapy – aquick review . Taiwan .J.Obst.Gyn., 48(3):239-244. DOI: 10.1016/S1028-4559(09)60296-5

Martin-B, Lucia,E.D et al.(2013). Biocompatible –Based Nanomedicine Engineered by Flow Focusing Efficient Antitumor Activity . International Journal of Pharmaceutics 443(1-2):103-9. DOI: 10.1016/j.ijpharm.2012.12.048

Minami, M., Mastsumoto, S., Horiuchi, H. (2010). Cardiovascular side effects of Modern cancer therapy .Cric.J.74(9): 1779-1786. DOI: 10.1253/circj.cj-10-0632

Osumi, J. and Hashimoto, T. (1978). Acyl-CoA oxidase of rat liver: A new enzyme for fatty acid oxidation. Biochem. Biophys. Res. Commun. 83, 479-485. DOI: 10.1016/0006-291x(78)91015-x

Radi, R., Beckman, J. S., Bush, K. M. and Freeman, B. A. (1991). Peroxynitrite oxidation of sulfhydryls. The cytosolic potential of superoxide and nitric oxide. J. Biol. Chem. 266: 4244-4250. https://doi.org/10.1016/S0021-9258(20)64313-7

Rodkey, F.L. (1965). Directed spectrophotomeric determination of albumin human serum.Clin. Chem. 1: 478.

Salami, Wajih Mazhar. (1998). Effect of extracts of the plant Convolvulus arvensis L. and Hendale lpomoae carrica (linn) on the biological performance of wheat bug Schizaphis graminu PhD thesis, College of Science / University of Babylon 111 pages.

Sanchez-Vioque, R., Rodrigues-Conde, M.F.,Reina-Urena, J.V., Escolano-Tercera, M. A.,Herraiz-Penalrer, D,Santana –Meridas, O (2012). In vitro antioxidant and metal chelating Properties of corm , petal and leaf from saffron ( Crocus sativus L.) Ind Crop Prod39:149- 153.

Schmidt, M., Betti, G., Hensel, A. (2007):Saffron in phytotherapy : pha- Rmacology and clinical use.Wien .Med.Wochenschr.,157:315- 319. DOI: 10.1007/s10354-007-0428-4

Silbergeld E, Waalkes M, Rice J.(2000). Lead as a carcinogen:Experimental evidence and mechanisms of action. Am J Indian Med. 38:316- 323.DOI: 10.1002/1097-0274(200009)38:3<316::aid-ajim11>3.0.co;2-p

Sudhakar, S., Seema, M., Rudra , D.T., Sanjay, D., Dharmendra, K.G.(1996). Copper-induced oxidative stress and responses of antioxidants and phytochelatins in Hydrilla verticillata (L.f.) Royle. Aquatic Toxicology.80(4): 405-415. DOI: 10.1016/j.aquatox.2006.10.006

Thakore, P., Mani, R.K., Singh, J., Kavitha, A.S.(2012). A brief review of plants Having anti-cancer property . Int J Pharm Res Dev 3:129-136.

Tousson, E., Zaki, T. Z., Walid, A. A., Hamada, H.(2014). Methotrexate-induced Hepatic and Renal Toxicity: Role of L-carnitine in Treatment. Biomedicine and Biotechnology, 2014, Vol. 2, No. 4, 85-92.

Upasani, C. and Balaraman, R.(2001). Effect of vitamin E, vitamin C and spirulina on levels of membrane bound enzymes and lipids in some organs of rats exposed to lead. Indian J Pharmacol. 33:185-191.

Veltkamp, S.A., Pluim, D., Van, T. O. Beijnen, J.H, Schellens, J.H.(2008). Extensive metabolism and hepatic accumulation of gemcitabine after multiple oral and intravenous administration in mice. Drug Metab Dispos .36:1606–15. DOI: 10.1124/dmd.108.021048

Zhao, J.A. , Peng, L., Geng, C.Z. , et al .(2014). Preventive effect of hydrazine curcumin on carcinogenesis of diethyl nitrosamine-induced hepatocarcinoma in male SD rats. Asian Pac J Cancer Prev, 15, 2115-21. DOI: 10.7314/apjcp.2014.15.5.2115

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Published

2023-09-07

How to Cite

Safa Masser kmosh, Masar J. AL-Kurdy, & Malath I. Yousif. (2023). Effect of Gemzar drug and plant extract of Crocus sativus L on some antioxidants in male rats. Czech Journal of Multidisciplinary Innovations, 21, 6–13. Retrieved from https://peerianjournal.com/index.php/czjmi/article/view/628

Issue

Vol. 21 (2023): CZJMI

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