Using the CRISPR-Cas9 System in Genetic Engineering Applications : A review Article

Main Article Content

Dr. Abdalkader Saeed Latif
Ammar Basim Al-Asadi
Dr. Mahmood Abed Hamzah
Dr. Reyam Naji Ajmi

Abstract

It has been described the CRISPR-Cas9 system ,the most exciting achievement in biomedical research since the dawn of genetic engineering in the 1970s, with the broad scope to better understand and treat human and animal diseases and its potential to revolutionize medical and agricultural research by identifying the double helix of DNA and the role it in the genetic formation of living organisms. The CRISPR tool is a giant step forward in this regard, and compared to existing research tools, it is a fast, easy, reliable, and relatively low-cost way to target and refine specific genetic sequences. Therefore, this paper discussed the importance of this technology, the way genes work in cells, and the most effective new medical treatments for a group disease that destroys the living body

Article Details

How to Cite
Dr. Abdalkader Saeed Latif, Ammar Basim Al-Asadi, Dr. Mahmood Abed Hamzah, & Dr. Reyam Naji Ajmi. (2023). Using the CRISPR-Cas9 System in Genetic Engineering Applications : A review Article. The Peerian Journal, 25, 74–85. Retrieved from https://peerianjournal.com/index.php/tpj/article/view/707
Section
Articles

References

IGI Forum on Bioethics, Napa, California; this meeting was sponsored by the Innovative Genomics Initiative at the University of California, Berkeley; 24 January 2015; University of California, San Francisco. all the authors, except for GC and MJ, participated in the meeting.

Abdalkader Saeed Latif, Zena Abbas Fadel. EVALUATION STUDY OF THE EFFECTIVENESS FOR SOME ANTIBACTERIAL AGENT AGAINST DNA GYRASE ENZYME OF STAPHYLOCOCCUS AUREUS. Eur. Chem. Bull. 2022,11(7), 29 – 32. DOI: 10.31838/ecb/2022.11.07.005. .

Yang, T.L. Ng, Fast Bayesian regression kriging method for real-time merging of radar, rain gauge, and crowd sourced rainfall data, Water Resources Research 55 (2019) 3194–3214.

Marraffini, L.A., and Sontheimer, E.J. (2008). CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA. Science 322, 1843–1845.

Zhu Z, González F, Huangfu D. Methods Enzymol. 2014;546:215. Medline.

Fadhel R, Zeki HF, Ati EM, Ajmi RN. Estimation Free Cyanide on the Sites Exposed of Organisms Mortality in Sura River /November 2018. Journal of Global Pharma Technology. Technology. (2019); 11(3) : 100-105

L. Gartland, Measuring And Modeling Heat Islands. Heat Islands: Understanding and Mitigating Heat In Urban Areas, Earthscan, London, UK, 2008, pp. 27–33.

Cho, S.W., Kim, S., Kim, J.M., and Kim, J.-S. (2013). Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat. Biotechnol. 31, 230–232.

Ferwati, C. Skelhorn, V. Shandas, J. Voelkel, A. Shawish, M. Ghanim, Analysis of urban heat in a corridor environment – The case of Doha, Qatar. Urban Climate 24 (2018) 692–702.

The Human Fertilisation and Embryology (Mitochondrial Donation) Regulations. 2015 www.legislation.gov.uk/uksi/2015/572/contents/made (effective 29 October 2015).

U.S. Food and Drug Administration, Cellular, Tissue, and Gene Therapies Advisory Committee Meeting:Announcement. www.fda.gov/AdvisoryCommittees/Calendar/ucm380042.htm; www.iom.edu/activities/research/mitoethics.aspx.

Sapranauskas, R., Gasiunas, G., Fremaux, C., Barrangou, R., Horvath, P., and Siksnys, V. (2011). The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res. 39, 9275–9282.

-Sharma, C.M., Hoffmann, S., Darfeuille, F., Reignier, J., Findeiss, S., Sittka, A., Chabas, S., Reiche, K., Hackermu¨ ller, J., Reinhardt, R.,. (2010). The primary transcriptome of the major human pathogen Helicobacter pylori. Nature 464, 250–255.

Abdalkader Saeed Latif, Alia Essam Mahmood Alubadi and Majida G. Magtooph. Computational study of Oseltamivir, Chloroquine, Hydroxy Chloroquine, Ribavirin and Kaletra against lysosomal protease of COVID19. International Journal of Psychosocial Rehabilitation. (2020). Vol. 24, Issue 05, PP 1170-1176. https://www.psychosocial.com/article/PR201792/16745/ .

Mali, P., Yang, L., Esvelt, K.M., Aach, J., Guell, M., DiCarlo, J.E., Norville, J.E., and Church, G.M. (2013). RNA-guided human genome engineering via Cas9. Science 339, 823–826.

Ajmi, R.N., Sultan, M., Hanno, S.H. Bioabsorbent of chromium, cadmium and lead from industrial waste water by waste plant. Journal of Pharmaceutical Sciences and Research, 2018, 10(3), pp. 672–674.

Gasiunas, G., Barrangou, R., Horvath, P., and Siksnys, V. (2012). Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc. Natl. Acad. Sci. USA 109, E2579–E2586.

Haber, J.E. (2000). Lucky breaks: analysis of recombination in Saccharomyces. Mutat. Res. 451, 53–69.

Hale, C.R., Zhao, P., Olson, S., Duff, M.O., Graveley, B.R., Wells, L., Terns, R.M., and Terns, M.P. (2009). RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex. Cell 139, 945–956.